EuroFlow PIDOT 2025
Primary ImmunoDeficiency Orientation Tube (PIDOT) EQA scheme
Aim of the scheme
The PIDOT scheme is divided into two parts. The aim of the wet lab part is to test the technical quality of your sample preparation and measurement on the flow cytometer when applying the EuroFlow PIDOT antibody panel and relevant EuroFlow standard operating procedures (SOPs). You will stain locally sourced healthy donor peripheral blood samples with the PIDOT antibody panel and analyze the data. The aim of the dry part is to evaluate your ability to analyze and interpret the FCS files provided. ESLHO will provide 2 FCS files acquired from patients with confirmed primary immunodeficiency (PID), non-PID disease controls in whom PID diagnosis was ruled out (as defined by the treating physician based on standard clinical care), or healthy donors. For both parts, you will report your results and conclusions via the ESLHO EQA Portal.
Your results will be compared to the reference results (Neirinck et al., 2024) and to the results of the other participants in the round.
Two unique rounds of the PIDOT scheme are offered in 2025: one in spring and one in autumn.
Performance and report
After each round, participating laboratories will be provided with an EQA certificate showing their performance as compared to the reference values and to other laboratories. In addition to the performance report, specific comments or recommendations on how routine practice could be improved may be provided.
Further details on data analysis, how participants’ performance is evaluated, and on the contents of the report can be found in the document “Scheme information” available on the Resources page.
Educational meeting
All EuroFlow schemes’ EQA rounds offered in 2025 will be concluded with an educational meeting, which will include all rounds performed throughout the year. Participation is free of charge for two representatives per participating laboratory. During the meeting, the rounds’ results will be shown (anonymized), possible problems and pitfalls will be discussed, and there will be the opportunity to receive direct feedback. More information regarding the educational meeting will be announced on the ESLHO EQA Portal.
Organization
The PIDOT scheme is organized by ESLHO in collaboration with the EuroFlow EQA Committee. The EuroFlow EQA Committee is composed of members of the EuroFlow Consortium (www.euroflow.org). The laboratory at the University of Ghent, Ghent, BE, operates as the lead expert laboratory with Prof. Dr. Carolien Bonroy in the role of lead subject-matter expert.
Further details on the task division of subject-matter experts can be found in the document “Scheme information” available on the Resources page.
Participation
ESLHO offers the PIDOT 2025 rounds free of charge to participants of the EuroFlow Consortium.